AFT™ Linked-Reads Single-Cell Whole-Genome Library preparation technology seamlessly integrates the Amplification, Fragmentation, and Tagging of target DNA from a single cell into one reaction, achieves ultimate efficiency in single cell DNA sequencing Library preparation. More significantly, reads are linked by native barcodes, enables long scaffolding , de novo assembly, and haploid phasing without compartmentation.
The simple workflow comprises of 3 functional steps: Single cell lysis, AFT reaction of one hour at 45°?C, and Library PCR of 16 cycles, there is also a SPRI beads-based purification before and after Library PCR step.
