AFT™ Linked-Reads Raw Blood WGS Library Preparation Kit seamlessly integrates the blood DNA extraction, Amplification, Fragmentation, and Tagging into one reaction, achieves ultimate efficiency in animal DNA sequencing Library preparation. All it takes is 1 uL of blood. More significantly, reads are linked by native barcodes, enables long scaffolding , de novo assembly, and haploid phasing without compartmentation.
The simple workflow comprises of 3 functional steps: blood cells lysis, AFT reaction of one hour at 45°?C, and Library PCR of 16 cycles, there is also a SPRI beads-based purification before and after Library PCR step.
