LyseNow(R) Strip Cards features proprietary lysis buffer impregnated filter paper discs that are assembled between two layers of transparent PET sheets and spaced out to align with one row of a 96 well plate format. The filter paper discs lyse cells or E. coli, or other host cells on contact, release plasmid DNA, pathogen DNA, or RNA for easy extraction; transparent card allows for the easy alignment of the discs over rows of 96 well plate, the discs can be effortlessly pushed down to 96 well plate wells with sterile pipette tips; nucleic acids from the host cells, plasmid DNA, pathogen DNA, or RNA, can then be easily eluted off with 10 minutes shaking. Elution can be used directly for PCR or sequencing. In one instance, more than 700 bp high quality sequencing data were generated with CE sequencing from lysates of overnight culture harboring a 14 kb low copy number plasmid. LyseNow(R) Strip Card produces little plastic waste and no any chemical waste at all. Up to 96 colonies can be processed for PCR ready DNA extraction in about 30 minutes.